Journal: eLife

Article Title: Interplay between PML NBs and HIRA for H3.3 dynamics following type I interferon stimulus

doi: 10.7554/eLife.80156

Figure Lengend Snippet:

Article Snippet: Human BJ primary foreskin fibroblasts (ATCC, CRL-2522), human IMR90 fetal lung fibroblasts (ATCC, CCL-186), human HEK 293T embryonic kidney cells (Intercell, AG) and mouse MEFs embryonic fibroblasts Pml +/+ or Pml -/- (from Dr. Lallemand-Breitenbach, and whose cell identity was authenticated by STR profiling) were cultivated in DMEM medium (Sigma-Aldrich, D6429) containing 10% of fetal calf serum (FCS) (Sigma-Aldrich, F7524), 1% of penicillin/streptomycin (Sigma-Aldrich, P4458), at 37 °C under 5% CO2 and humid atmosphere.

Techniques: Transduction, Recombinant, Plasmid Preparation, Clone Assay, Mutagenesis, Sequencing, Quantitative RT-PCR, Concentration Assay, In Situ, Software, ChIP-sequencing

Journal: The Journal of Biological Chemistry

Article Title: Modeling protease-sensitive human pancreatic lipase mutations in the mouse ortholog

doi: 10.1016/j.jbc.2024.107763

Figure Lengend Snippet: Secretion of mouse PNLIP variants. A , PNLIP protein levels in the conditioned media of transiently transfected HEK 293T cells were analyzed by reducing SDS-PAGE and Coomassie staining ( upper panel). PNLIP protein levels in the total cell lysates were determined by SDS-PAGE and western blotting ( lower panel). The levels of GAPDH were also assessed as controls. B , densitometric evaluation of PNLIP band intensities in the conditioned media and cell lysates; and assessment of PNLIP enzyme activities in the conditioned media. Samples for all the experiments were collected 48 h after transfection. Mean ± SD of 3 to 5 independent biological replicates are shown. Note that some data points exhibit overlap. ∗ p ≤ 0.05; ∗∗∗ p ≤ 0.001.

Article Snippet: Human Embryonic Kidney (HEK) 293T cells (Merck) were cultured and grown overnight in 2 ml of high-glucose DMEM cell culture media containing 10% FBS, 4 mM glutamine, 50 U/ml penicillin, and 50 μg/ml streptomycin in a 6-well tissue culture plate (1.2 million/well).

Techniques: Transfection, SDS Page, Staining, Western Blot